Aqp3 sirna
WebCombination of AQP5-siRNA and AQP3-siRNA. Combined treatment of AQP5-siRNA and AQP3-siRNA was performed. Briefly, after the low concentration of AQP5-siRNA (20nM) and AQP3-siRNA (20 nM) treatment for 24 h, the medium was changed to DMEM containing 10% FBS. Then, viable cell number was measured 48 h after the medium … WebFigure 5. Nicotinamide (Nico) inhibits all-trans retinoic acid (atRA) induced water permeability mediated by AQP3 in cultured human skin keratinocytes. HaCaT cells were treated with atRA (1.0 μM) for 24 h with or without Nico (100 μM) pretreatment for 1 h. Cell water permeability was analyzed by the method described in Materials and methods …
Aqp3 sirna
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Web22 apr 2024 · AQP3 siRNA could significantly downregulate AQP3 mRNA expression in the proliferating porcine intramuscular preadipocytes 24 h post-transfection (Figure 4A), … Web11 mag 2016 · Aquaporin-3 (AQP3) is a small transmembrane water/glycerol channel that may facilitate the membrane uptake of hydrogen peroxide (H2O2). Here we report that …
Webin AQP3 siRNA group (vs. scrambled siRNA group), and 66.9% in AQP3 siRNA+ E 2 group (vs. scram - bled siRNA+ E 2 group). As shown in Fig. 4E,F, knockdown of AQP3 significantly reduced 43.2% of the Web8 apr 2024 · Methods. We obtained human AQP3 sequence from the Genbank and established the recombinant vector expressing siRNA targeting AQP3.After the transfection of the recombinant vectors, the expression of AQP3 was determined by RT-PCR and western blot. The MTS assay, flow cytometry and Transwell assay were conducted to …
WebAQP3. AQP3 was the first aquaglyceroporin known and studied in humans and is expressed in a variety of tissues, including the renal collecting duct [], respiratory epithelium [], breast [], stomach [], and prostate [].Recently, an increasing number of researchers have pointed out that AQP3 is inclined to be of considerable importance in cancer development, which … Web14 mar 2011 · Combination of AQP3 siRNA and anti‐tumor drug. Combined treatment of AQP3‐siRNA with an anti‐tumor drug, cisplatin, was performed. Briefly, after the low concentration of AQP3‐siRNA (20 nM) treatment, 5 μM of cisplatin, which was a slightly effective concentration on cell growth inhibition, was treated for 48 h.
Web22 apr 2024 · Furthermore, deletion of AQP3 by siRNA significantly downregulated expression of cell cycle genes (cyclin D, E), and decreased the number of EdU-positive cells as well as cell viability. Collectively, our data indicate that AQP3 is of great importance in both adipogenic differentiation and proliferation in intramuscular adipocytes, providing a …
Web28 mag 2024 · We have previously shown that in mouse keratinocytes AQP3 protein levels and mRNA expression are decreased by differentiating agents, including 1,25 … ck diver\u0027sWeb包含至少一个检索词. 不包含检索词. 出现检索词的位置 ck dramatist\u0027sWeb20 apr 2024 · AQP3, in contrast to most members of the water channel aquaporin (AQP) family, can transport other small molecules, such as glycerol and H2O2, important for the physiological and pathological balance of hydrostatic and osmotic pressures in the plasma membrane [ 30 ]. ckd marijuanaWeb23 giu 2024 · AQP3 siRNA and PLD2 siRNA significantly downregulated the mRNA and protein levels of AQP3 and PLD2 in the A431 cells; inhibiting proliferation and promoting … ck djWeb22 ott 2024 · In animals and plants, membrane vesicles containing proteins have been defined as key for biological systems involving different processes such as trafficking or intercellular communication. Docking and fusion of vesicles to the plasma membrane occur in living cells in response to different stimuli, such as environmental changes or … ckd trnava.skWeb29 lug 2015 · As shown in Fig. 4C,D, knockdown of AQP3 decreased 29.7% of the migration distance in AQP3 siRNA group (vs. scrambled siRNA group) and 66.9% in AQP3 siRNA + E 2 group ... ck donji ves muskiWeb22 apr 2024 · Aquaglyceroporin (AQP) defines a class of water/glycerol channels that primarily facilitate the passive transport of glycerol and water across biological … ckd kogata