Phi x174 bacteriophage
WebbUnlike many bacteriophages, (φ)X174 does not utilize a proteinaceous host cell receptor for attachment and DNA translocation. Lipopolysaccharide (LPS) structure is both necessary and sufficient for host cell susceptibility. Recognition involves three viral proteins. A six-carbon sugar-binding site is located in the coat protein.
Phi x174 bacteriophage
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WebbWe present a screening procedure for classifying bacteriophage phi X174 mutants as defective in either "early" (genome delivery and RF DNA replication) or "late" (progeny ssDNA replication and morphogenesis) functions. When applied to 108 ts mutants, only five were classified as "early" mutants. Qua … Webb1 aug. 1992 · The structure of the single-stranded DNA phage phiX174 has been determined to 3.4 A resolution. The crystal space group was P2 (1) with one icosahedral …
Webb11 dec. 2024 · Manage alerts. Tailed bacteriophages use their tail complexes for host cell recognition, adsorption, and penetration of bacterial cell walls ( 1 – 4 ). However, it remains unclear how tailless phages like ΦX174 perform tail-associated functions. ΦX174 is a small icosahedral, tailless bacteriophage with a maximum diameter of ∼320 Å. WebbA histidine-tagged G protein of bacteriophage phi X174 (HisG) bound strongly with lipopolysaccharide (LPS) of Escherichia coli C, one of a phi X174-sensitive Ra strain. The dissociation constant, Kd, was measured to be 0.16 +/- 0.04 microM by fluorometric titration. HisG showed slightly less affinity to LPSs of the insensitive Rc and Rd 2 strains …
WebbThe phage ϕX174 was the first organism to have its entire nucleotide sequence determined, a feat that was accomplished by Frederick Sanger and colleagues in 1977. In the 1980s American biochemist George P. … WebbNature Previously we have developed an in vitro system that synthesizes infectious single-stranded (ss) DNA bacteriophage ΦX174 from purified phage components1. This system provides an assay to examine the function of each viral component in the phage synthesizing reaction.
WebbDer Enterobakteriophage PhiX174, offiziell Escherichia virus phiX174, veraltet Enterobacteria phage phiX174, deutsch auch Bakteriophage Phi X 174 oder Coliphage …
Webb업무개요 * 항균시험 - 기능성 소재 및 건축자재, 다용도소독기, 방역살균기 등에 대한 항균력 - 시험방법 : astm e2315, kcl-fir-1002, en iso 20743, jis z 2801, kcl-fir-1003, ks l iso 27447, 의뢰자제시 - 시험균주 : 대장균, 녹농균, 황색포도상구균, 폐렴균, … how to remove fullscreenWebbThe phiX174 bacteriophage attacks the common human bacteria Escherichia coli, infecting the cell and forcing it to make new viruses. Do you think that viruses are living … nordstrom tax service saginaw miWebbBacteriophage phi-X174 and related viruses. Bacteriophage phi-X174 and related viruses Prog Nucleic Acid Res Mol Biol. 1968;8:115-69. Author R L Sinsheimer. PMID: 4874231 … how to remove full directory in linuxWebbEnterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) Taxonomic identifier 1217068 NCBI Taxonomic lineage Viruses > Monodnaviria > Sangervirae > Phixviricota > Malgrandaviricetes > Petitvirales > Microviridae > Bullavirinae > Sinsheimervirus Virus hosts Escherichia coli C Accessions Primary accession P03639 … how to remove funds from inkstoneThe phi X 174 (or ΦX174) bacteriophage is a single-stranded DNA (ssDNA) virus that infects Escherichia coli, and the first DNA-based genome to be sequenced. This work was completed by Fred Sanger and his team in 1977. In 1962, Walter Fiers and Robert Sinsheimer had already demonstrated the physical, … Visa mer This bacteriophage has a [+] sense circular single-stranded DNA genome of 5,386 nucleotides. The genome GC-content is 44% and 95% of nucleotides belong to coding genes. Because of the balance base pattern of the … Visa mer Infection begins when G protein binds to lipopolysaccharides on the bacterial host cell surface. H protein (or the DNA Pilot Protein) pilots the … Visa mer Experimental evolution ΦX174 has been used as a model organism in many evolution experiments. Biotechnology ΦX174 is regularly … Visa mer • Goodsell D (February 2000). "Bacteriophage phiX174". Molecule of the Month. RCSB-PDB. Visa mer ΦX174 is closely related to other microviridae, especially the NC phage (e.g. NC1, NC7, NC11, NC16, NC37, NC5, NC41, NC56, NC51, etc.) and more distantly related to the G4-like phages and even more distantly related to the α3-like phage. Rokyta et al. 2006 … Visa mer • Viruses portal • Bacteriophage MS2 Visa mer how to remove fujitsu indoor unit coverWebbThe Phi-X174 bacteriophage was the very first organism to have the whole genome sequenced in 1977. Various research groups began sequencing DNA, with Fredrick … how to remove functionWebb24 aug. 2024 · Following incubation at 37 °C for 18 h, the growth of bacteria to an opaque, confluent layer was observed in areas in which the Phi X174 or Phi 44AHJD phage was not present. However, in the zone in which the Phi X174 or Phi 44AHJD bacteriophage was present, a consistent infection of the host cells by the bacteriophage progenies … how to remove functionloginput